Formalin-fixed and paraffin-embedded human hepatocarcinoma with PIGR Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
PIGR Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human colon tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Confocal immunofluorescent of PIGR Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot of PIGR Antibody in HepG2(lane 1),K562(lane 2),HL-60(lane 3) cell line lysates (35 ug/lane). PIGR (arrow) was detected using the purified antibody.
Flow cytometric of HepG2 cells using PIGR Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.