Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PCNA antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent of PCNA Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Western blot of PCNA Antibody in Jurkat(lane 1), HeLa(lane 2), 293(lane 3) cell line lysates (35 ug/lane). PCNA (arrow) was detected using the purified antibody.
PCNA Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.