Phospho-p53 (Ser15) Antibody

Name	Phospho-p53 (Ser15) Antibody
Supplier	Cell Signaling Technology
Catalog	9284
Prices	$109.00, $287.00, $678.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host	Rabbit
Clonality	Polyclonal
Applications	WB IP ChIP
Species Reactivities	Human, Mouse, Rat, Monkey, Mink, Bovine, Pig
Antigen	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser15 of human p53
Description	Rabbit Polyclonal
Gene	TP53
Supplier Page	Shop

Product images

Western blot analysis of extracts from MvILu cells treated with UV or hydroxyurea (20 mM) for the indicated times, using Phospho-p53 (Ser15) Antibody.

Western blot analysis of a p53 fusion protein, untreated or phosphorylated by DNA-PK, using Phospho-p53 (Ser15) Antibody (upper) and p53 Antibody #9282 (lower).

Western blot analysis of extracts from PC12 cells treated with UV for the indicated times, using Phospho-p53 (Ser15) Antibody.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HCT116 cells treated with UV (100 J/m 2 followed by a 3 hour recovery) and either 5 μl of Phospho-p53 (Ser15) Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human CDKN1A Promoter Primers #6449, human MDM2 intron 2 primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.