Detection of Human P2RX5 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-P2RX5 antibody used for IP at 6 ug per reaction. P2RX5 was also immunoprecipitated by rabbit anti-P2RX5 antibody BL16575. For blotting immunoprecipitated P2RX5, was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human P2RX5 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-P2RX5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.