Fluorescent confocal image of HeLa cell stained with NR3C1 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR3C1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NR3C1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.

NR3C1 Antibody western blot of mouse liver tissue lysates (35 ug/lane). The NR3C1 antibody detected NR3C1 protein (arrow).