Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb

Name	Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb
Supplier	Cell Signaling Technology
Catalog	13346
Prices	$287.00
Sizes	100 µl (10 western blots)
Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b
Clone	E1Z1T
Applications	WB IP
Species Reactivities	Human, Mouse, Rat
Antigen	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-κB protein.
Description	Mouse Monoclonal
Gene	RELA
Supplier Page	Shop

Product images

Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight), untreated (-) or LPS-treated (1 μg/ml, 15 min; +), using Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb (upper) or NF-κB p65 (D14E12) XP ® Rabbit mAb #8242 (lower).

Western blot analysis of extracts from HeLa and PC-3 cells, untreated (-) or treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (hTNF-α; 20 ng/ml, 5 min; +), using Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb (upper) or NF-κB p65 (D14E12) XP ® Rabbit mAb #8242 (lower).

Immunoprecipitation of phospho-NF-κB p65 (Ser536) from HeLa cells treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (20 ng/ml, 5 min) using Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) or Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb (lane 3). Lane 1 represents 10% input. Western blot was performed using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033.