Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb

Name	Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
Supplier	Cell Signaling Technology
Catalog	3033
Prices	$109.00, $287.00, $678.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	93H1
Applications	WB IP ICC/IF FC
Species Reactivities	Human, Mouse, Rat, Hamster, Monkey, Pig, Dog
Antigen	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-κB p65.
Description	Rabbit Monoclonal
Gene	RELA
Supplier Page	Shop

Product images

Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or TNF-α treated (#2169, 20 ng/ml for 5 minutes), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (upper) or NF-κB p65 Antibody #3034 (lower).

Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24h) and treated with 1 μg/ml LPS for the indicated times, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (upper) and NF-κB p65 (C22B4) Rabbit mAb #4764 (lower).

Confocal immunofluorescent analysis of HeLa cells, serum starved (left) or TNF-α treated (#8902 at 20 ng/ml for 20 min, right), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® phalloidin 555 (red).

Flow cytometric analysis of HeLa cells, untreated (blue) or TNF-α-treated (green), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb compared to a nonspecific negative control antibody (red).