
Immunostaining analysis in HT1080 cells. HT1080 cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-NARS mAb.

Western blot analysis of immunized recombinant protein, using anti-NARS monoclonal antibody.

Detection of NARS by Western blot. Samples: Whole cell lysate from human HT1080 (H, 25 ug) and mouse NIH3T3 (M, 25 ug) cells. Predicted molecular weight: 62 kDa

Immunoprecipitation: RIPA lysate of HT1080 cells was incubated with anti-NARS mAb. Predicted molecular weight: 62 kDa