
p-4E-BP1/2/3 (Thr 45)-R: sc-18090-R. Western blot analysis of 4E-BP1 phosphorylation in non-transfected 293T: sc-117752 (A) and human 4E-BP1 transfected 293T: sc-116590 (B) whole cell lysates and HeLa nuclear extract (C).

p-4E-BP1/2/3 (Thr 45)-R: sc-18090-R. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A) and untreated human 4E-BP1 transfected: sc-116590 (B) 293T whole cell lysates.

p-4E-BP1/2/3 (Thr 45)-R: sc-18090-R. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A), untreated human 4E-BP1 transfected: sc-116590 (B) and lambda protein phosphatase treated human 4E-BP1 transfected: sc-116590 (C) 293T whole cell lysates.

Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A,D), untreated human 4E-BP1 transfected: sc-116590 (B,E) and lambda protein phosphatase (sc-200312A) treated human 4E-BP1 transfected: sc-116590 (C,F) 293T whole cell lysates. Antibodies tested include p-4E-BP1/2/3 (Thr 45)-R: sc-18090-R (A,B,C) and 4E-BP1 (11G12C11): sc-81149 (D,E,F).

Western blot analysis of 4E-BP1/2/3 phosphorylation in untreated (A), calyculin A treated (B) and calyculin A and lambda protein phosphatase (sc-200312A) treated (C) Jurkat whole cell lysates. Antibodies tested include p-4E-BP1/2/3 (Thr 45)-R: sc-18090-R (A,B,C).