Anti-MAPKAPK2 / MK2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10396 concentration 5 ug/ml.
MAPKAPK2 antibody and EB11930 (1.5 ug) immunoprecipitations from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labeled with rabbit anti-MK2.
MAPKAPK2 antibody (0.5 ug/ml) staining of MEF lysates (35 ug protein in RIPA buffer), from double KO mice in second and third lanes and rescued by introduction of MK2 gene in second lane. Primary incubation was 2 hour. Detected by chemiluminescence.
MAPKAPK2 antibody HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with (0.5 ug/ml) in the left panel and with EB11930 (0.5 ug/ml) in the right panel.