Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ERK8 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of ERK8 (arrow) using rabbit polyclonal ERK8 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK15 gene (Lane 2) (Origene Technologies).
Western blot of anti-ERK8 Antibody in T47D cell line lysates (35 ug/lane). ERK8 (arrow) was detected using the purified antibody.