Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of MAPK12 (arrow) using rabbit polyclonal hMAPK12-P354. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPK12 gene.
Western blot of p38 gamma (MAPK12) Antibody in mouse lung tissue lysates (35 ug/lane). MAPK12 (arrow) was detected using the purified antibody.