Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Fluorescent image of U251 cell stained with MEK2 (MAP2K2) Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). MEK2 immunoreactivity is localized to Cytoplasm significantly.
MAP2K2 Antibody (A278) western blot of U937 cell line lysates (35 ug/lane). The MAP2K2 antibody detected the MAP2K2 protein (arrow).
The anti-MAP2K2 antibody is used in Western blot to detect MAP2K2 in mouse liver tissue lysate.
Western blot of MAP2K2 (arrow) using MAP2K2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2) (Origene Technologies).