Immunocytochemical staining of a rat cardiac fibroblast using anti-Lipoma Preferred Partner, pAb (IG-817) (LPP; red fluorescence) and a green-fluorescent phalloidin derivative staining filamentous actin. LPP is a protein related to the cytoskeletal and focal adhesion protein zyxin.
Western blot of total human skin fibroblast proteins using anti-Lipoma Preferred Partner, pAb (IG-817) . Total cell protein (25ug) was separated by SDS-PAGE and blotted onto nitrocellulose. The blot was incubated with 0.5ug/ml of anti-Lipoma Preferred Partner, pAb (IG-817) followed by 125I-protein A.