![Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells, treated with either hydroxyurea (4 mM, 20 hr; +) to induce G1/S phase or Paclitaxel #9807 (100 nM, 20 hr; +) to induce G2/M phase, using Phospho-Lamin A/C (Ser22) (D2B2E) XP ® Rabbit mAb (upper) and Lamin A/C Antibody #2032 (lower).](http://www.bioprodhub.com/system/product_images/ab_products/1/sub_1/24765_13448_gel_lg.jpg)
Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells, treated with either hydroxyurea (4 mM, 20 hr; +) to induce G1/S phase or Paclitaxel #9807 (100 nM, 20 hr; +) to induce G2/M phase, using Phospho-Lamin A/C (Ser22) (D2B2E) XP ® Rabbit mAb (upper) and Lamin A/C Antibody #2032 (lower).
![Immunoprecipitation of phospho-Lamin A/C (Ser22) from extracts of HeLa cells treated with Paclitaxel #9807, using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Phospho-Lamin A/C (Ser22) (D2B2E) X ® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Lamin A/C (Ser22) (D2B2E) XP ® Rabbit mAb.](http://www.bioprodhub.com/system/product_images/ab_products/1/sub_1/24766_13448_ip_lg.jpg)
Immunoprecipitation of phospho-Lamin A/C (Ser22) from extracts of HeLa cells treated with Paclitaxel #9807, using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Phospho-Lamin A/C (Ser22) (D2B2E) X ® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Lamin A/C (Ser22) (D2B2E) XP ® Rabbit mAb.
![Confocal immunofluorescent analysis of HeLa cells, untreated (left) or hydroxyurea-treated (right), using Phospho-Lamin A/C (Ser22) (D2B2E) XP ® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).](http://www.bioprodhub.com/system/product_images/ab_products/1/sub_1/24767_13448_if_lg.jpg)
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or hydroxyurea-treated (right), using Phospho-Lamin A/C (Ser22) (D2B2E) XP ® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).