Anti-HLA-G antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10545 dilution 1:100.
Western blot of HLA-G (arrow) using rabbit polyclonal HLA-G Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-G gene.
Western blot of HLA-G Antibody in NCI-H460 cell line lysates (35 ug/lane). HLA-G (arrow) was detected using the purified antibody.
Western blot of lysate from human placenta tissue lysate, using HLA-G Antibody ). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
HLA-G Antibody flow cytometry of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.