Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HLA-B Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of HLA-B Antibody in A375 cell line lysates (35 ug/lane). HLA-B (arrow) was detected using the purified antibody.
Western blot of HLA-B (arrow) using rabbit polyclonal HLA-B Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-B gene.
Western blot of lysates from CEM, HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot of lysates from HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot of HLA-B Antibody in A2058 cell line lysates (35 ug/lane). HLA-B (arrow) was detected using the purified antibody.
HLA-B Antibody flow cytometry of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.