Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Detection of interaction between HIPK3 and SF-1 by coimmunoprecipitation. After expression of SF-1-HA and Flag-sHIPK3 (aa 159 to 1191) in H1299 cells, the HIPK3 protein complex was immunoprecipitated with anti-Flag antibody or by direct loading to the gel (input). Western blotting was then performed to detect SF-1-HA and Flag-sHIPK3.