IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
IHC of paraffin-embedded Human pancreas tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunofluorescent staining of HT29 cells using anti-LGR5 mouse monoclonal antibody (green). Actin filaments were labeled with TRITC-phalloidin (Red), and nuclear with DAPI (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5.