GPER in Human Brain. GPER was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Goat Anti-Human GPER Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml overnight at 4°C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies.
Detection of Human GPER by Western Blot. Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human GPER Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for GPER at approximately 54 kD (as indicated). This experiment was conducted under reducing conditions.