RM260 specifically reacts to Histone H2B acetylated
at Lysine 23 (K23ac). No cross reactivity with acetyl-
ated Lysine 5 (K5ac), Lysine 11 (K11ac), Lysine 12
(K12ac), Lysine 15 (K15ac), Lysine 20 (K20ac), or
non-mordified Lysine 23 in histone H2B.
Western Blot of (1) acid extracts of HeLa cells treated
with sodium butyrate, (2) acid extracts of HeLa cells
untreated, and (3) recombinant Histone H2B, using
Acetyl-Histone H2B (Lys23) rabbit monoclonal antibody
(RM260) at 0.5 ug/mL.
Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H2B (Lys23)
rabbit monoclonal antibody (RM260) (red). Actin
filaments have been labeled with fluorescein
phalloidin (green).