
Formalin-fixed and paraffin-embedded human brain tissue reacted with FTO Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot of FTO Antibody in NCI-H460 cell line lysates (35 ug/lane). FTO (arrow) was detected using the purified antibody.

Flow cytometric of NCI-H292 cells using FTO Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.