
Raf-1 (C-20): sc-227. Immunofluorescence staining of methanol-fixed 3611-RF cells showing cytoplasmic staining.

Western blot analysis of Raf-1 expression in KNRK (A), NIH/3T3 (B), 3611-RF (C,E) and HeLa (D) whole cell lysates. Antibodies tested include Raf-1 (C-12): sc-133 (A-D) and Raf-1 (C-20): sc-227 (E).

Raf-1 (C-20): sc-227. Western blot analysis of Raf-1 expression in untreated (A) and serum-starved, PMA-treated HeLa (B) and untreated (C) and PDGF-treated CCD-1064Sk (D) whole cell lysates.

Raf-1 (C-20): sc-227. Western blot analysis of Raf-1 expression in non-transfected 293T: sc-117752 (A), mouse Raf-1 transfected 293T: sc-122942 (B) and HeLa (C) whole cell lysates.

Raf-1 (C-20)-G: sc-227-G. Western blot analysis of Raf-1 expression in non-transfected: sc-117752 (A) and mouse Raf-1 transfected: sc-122942 (B) 293T whole cell lysates.

Raf-1 (C-20): sc-227. Western blot analysis of Raf-1 expression in non-transfected 293: sc-110760 (A), human Raf-1 transfected 293: sc-158911 (B) and HeLa (C) whole cell lysates.

Raf-1 (C-20): sc-227. Western blot analysis of Raf-1 expression in non-transfected: sc-117752 (A) and human Raf-1 transfected: sc-113164 (B) 293T whole cell lysates.

Raf-1 (C-20)-G: sc-227-G. Western blot analysis of Raf-1 expression in non-transfected: sc-117752 (A) and human Raf-1 transfected: sc-113164 (B) 293T whole cell lysates.

Western blot analysis of Raf-1 phosphorylation in non-transfected: sc-117752 (A,D), untreated mouse Raf-1 transfected: sc-122942 (B,E) and lambda protein phosphatase (sc-200312A) treated mouse Raf-1 transfected: sc-122942 (C,F) 293T whole cell lysates. Antibodies tested include p-Raf-1 (H-8): sc-271928 (A,B,C) and Raf-1 (C-20): sc-227 (D,E,F).

Western blot analysis of Raf-1 phosphorylation in non-transfected: sc-117752 (A,D), untreated mouse Raf-1 transfected: sc-122942 (B,E) and lambda protein phosphatase (sc-200312A) treated mouse Raf-1 transfected: sc-122942 (C,F) 293T whole cell lysates. Antibodies tested include p-Raf-1 (E-1): sc-271929 (A,B,C) and Raf-1 (C-20): sc-227 (D,E,F).

Western blot analysis of Raf-1 phosphorylation in untreated (A,D), serum starved and PMA treated (B,E) and serum starved, PMA treated and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-Raf-1 (DPR/2/2A/3A): sc-57555 (A,B,C) and Raf-1 (C-20): sc-227 (D,E,F).

Western blot analysis of Raf-1 phosphorylation in untreated (A,D), serum starved and PMA treated (B,E) and serum starved, PMA treated and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-Raf-1 (Ser 338/Tyr 341)-R: sc-28005-R (A,B,C) and Raf-1 (C-20): sc-227 (D,E,F).

Raf-1 (C-20)-G: sc-227-G. Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing nuclear staining of respiratory epithelial cells.