
Western blot of extracts from A431 cells,untreated or treated with EGF using Phospho-EGFR(Tyr1172)(left) or EGFR antibody(right).

Western blot of EGFR (arrow) in HeLa cell lysates, either induced (Lane 1) or non-induced with EGF (Lane 2).

Western blot of extracts from A431 cells, untreated or treated with EGF, using phospho EGFR-Y1172 (left) or ErBB2 antibody (right)

Dot blot of anti-EGFR-pY1172 Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.