Fluorescent image of A549 cell stained with FADS2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FADS2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). FADS2 immunoreactivity is localized to Cytoplasm and Vesicles significantly.

FADS2 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The FADS2 antibody detected the FADS2 protein (arrow).

FADS2 Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.