Histone H2B (D2H6) Rabbit mAb

Name	Histone H2B (D2H6) Rabbit mAb
Supplier	Cell Signaling Technology
Catalog	12364
Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	D2H6
Applications	WB IHC-P ChIP
Species Reactivities	Human, Mouse, Rat, Monkey, Chicken, Zebrafish, Bovine
Antigen	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val118 of human histone H2B protein.
Description	Rabbit Monoclonal
Gene	HIST1H2BB
Supplier Page	Shop

Product images

Western blot analysis of extracts from various cell lines using Histone H2B (D2H6) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse prostate using Histone H2B (D2H6) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Histone H2B (D2H6) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Histone H2B (D2H6) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells and either 10 μl of Histone H2B (D2H6) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human GAPDH Exon 1 Primers #5516, SimpleChIP ® Human RPL30 Exon 3 Primers #7014, SimpleChIP ® Human MYT-1 Exon 1 Primers #4493, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.