Ubiquityl-Histone H2B (Lys120) (D11) XP ® Rabbit mAb

Name	Ubiquityl-Histone H2B (Lys120) (D11) XP ® Rabbit mAb
Supplier	Cell Signaling Technology
Catalog	5546
Prices	$119.00, $318.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	D11
Applications	WB ICC/IF FC ChIP
Species Reactivities	Human, Mouse, Rat, Monkey
Antigen	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human histone H2B protein in which Lys120 is mono-ubiquitylated.
Description	Rabbit Monoclonal
Gene	HIST1H2BB
Supplier Page	Shop

Product images

Western blot analysis of extracts from various cell lines using Ubiquityl-Histone H2B (Lys120) (D11) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of HeLa cells using Ubiquityl-Histone H2B (Lys120) (D11) XP ® Rabbit mAb (green) and COX IV (4D11-B3-E8) Mouse mAb #11967 (red).

Flow cytometric analysis of HeLa cells using Ubiquityl-Histone H2B (Lys120) (D11) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells and either 5 μl of Ubiquityl-Histone H2B (Lys120) (D11) XP ® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human γ-Actin Promoter Primers #5037, SimpleChIP ® Human γ-Actin Intron 3 Primers #5047, SimpleChIP ® Human GAPDH Promoter Primers #4471, and SimpleChIP ® Human GAPDH Intron 2 Primers #4478. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.