Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated)
| Name | Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated) |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 9072 |
| Prices | $287.00 |
| Sizes | 100 µl (10 immunoprecipitations) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | D7H4 |
| Applications | IP ChIP |
| Species Reactivities | Human, Mouse, Rat, Monkey, Chicken, Xenopus, Zebrafish, Bovine, Pig, Horse |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys12 of human histone H2B protein. |
| Description | Rabbit Monoclonal |
| Gene | HIST1H2BB |
| Conjugate | ChIP Formulated |
| Supplier Page | Shop |
Product images
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells and either 10 µl of Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated) or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human GAPDH Exon 1 Primers #5516, SimpleChIP ® Human RPL30 Exon 3 Primers #7014, SimpleChIP ® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.