Western blot analysis of extracts from various cell lines, untreated or TSA-treated (400 nM for 12 hours), using Acetyl-Histone H2B (Lys20) Antibody.
Immunohistochemical staining of paraffin-embedded human breast carcinoma, showing nuclear localization of histone H2B, using Acetyl-Histone H2B (Lys20) Antibody (left), or the same antibody preincubated with specific acetyl histone H2B peptide (right).
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Trichostatin A (TSA) #9950 (1 μM for 24 hours; right) using Acetyl-Histone H2B (Lys20) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red).