Acetyl-Histone H2B (Lys5) (D5H1S) XP ® Rabbit mAb

Name	Acetyl-Histone H2B (Lys5) (D5H1S) XP ® Rabbit mAb
Supplier	Cell Signaling Technology
Catalog	12799
Prices	$119.00, $318.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	D5H1S
Applications	WB IP IHC-P ICC/IF ChIP
Species Reactivities	Human, Mouse, Rat, Monkey, Hamster, Chicken, Zebrafish, Bovine, Horse
Antigen	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding acetylated Lys5 of human histone H2B protein.
Description	Rabbit Monoclonal
Gene	HIST1H2BB
Supplier Page	Shop

Product images

Western blot analysis of extracts from HeLa and C6 cells, untreated (-) or treated with Trichostatin A (TSA) #9950 (1 μM, 18hr; +), using Acetyl-Histone H2B (Lys5) (D5H1S) XP ® Rabbit mAb (upper) or Histone H2B (D2H6) Rabbit mAb #12364 (lower).

Immunoprecipitation of acetylated histone H2B from HeLa cell extracts treated with Trichostatin A (TSA) #9950 (1 μM, 18hr) using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Acetyl-Histone H2B (D5H1S) XP ® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Acetyl-Histone H2B (D5H1S) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Acetyl-Histone H2B (Lys5) (D5H1S) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Acetyl-Histone H2B (Lys5) (D5H1S) XP ® Rabbit mAb in the presence of non-acetyl peptide (left) or Lys5 acetyl peptide (right).

Confocal immunofluorescent analysis of HeLa cells, untreated (right) or treated with Trichostatin A (TSA) #9950 (left), using Acetyl-Histone H2B (Lys5) (D5H1S) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells and either 10 µl of Acetyl-Histone H2B (Lys5) (D5H1S) XP ® Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human GAPDH Exon 1 Primers #5516, SimpleChIP ® Human RPL30 Exon 3 Primers #7014, SimpleChIP ® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.