Hydroxy-HIF-1α (Pro564) (D43B5) XP ® Rabbit mAb
| Name | Hydroxy-HIF-1α (Pro564) (D43B5) XP ® Rabbit mAb |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 3434 |
| Prices | $119.00, $318.00 |
| Sizes | 20 µl (2 western blots), 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | D43B5 |
| Applications | WB IP ICC/IF |
| Species Reactivities | Human, Monkey, Mouse, Rat, Chicken, Xenopus, Zebrafish, Pig |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic hydroxypeptide corresponding to residues surrounding Pro564 of human HIF-1α. |
| Description | Rabbit Monoclonal |
| Gene | HIF1A |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from HeLa cells, treated with either 10 μM of MG132 (to accumulate hydroxylated HIF-1α) or 10 µM MG132 and 1 mM DMOG (to accumulate nonhyroxylated HIF-1α), using Hydroxy-HIF-1α (Pro564) (D43B5) XP ® Rabbit mAb (upper) or total HIF-1α Antibody #3716 (lower).
Confocal immunofluorescent analysis of HeLa cells, treated with either 10 μM MG132 (left) or 10 μM MG132 and 1 mM DMOG (right), using Hydroxy-HIF-1α (Pro564) (D43B5) XP ® Rabbit mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red).