Immunohistochemistry Analysis:
Representative lot data.
Formalin Fixed Paraffin Embedded (FFPE) mouse liver (Fig. 1) and mouse cardiac muscle (Fig. 2) tissues were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ABD75, THAP11 (Ronin). Reactivity was detected using an Anti-Rabbit secondary antibody and HRP-DAB. Positive nuclear and cytoplasmic staining was observed in both mouse liver and heart sections.
Western Blotting Analysis:
Representative lot data.
Mouse heart tissue lysate were probed with Cat. No. ABD75, THAP11 (Ronin) (1 µg/mL) .
Proteins were visualized using a Donkey Anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates Thap11 (~ 48 kDa). Note: Uncharacterized bands appear at ~20-25 kDa in some lysates.
Western Blotting Analysis:
Representative lot data.
Mouse liver tissue lysate were probed with Cat. No. ABD75, THAP11 (Ronin) (1 µg/mL) .
Proteins were visualized using a Donkey Anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates Thap11 (~ 48 kDa). Note: Uncharacterized bands appear at ~20-25 kDa in some lysates.