Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb

Name	Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb
Supplier	Cell Signaling Technology
Catalog	5558
Prices	$119.00, $318.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	D85E12
Applications	WB IP ICC/IF FC
Species Reactivities	Human, Mouse, Rat, Hamster
Antigen	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser9 of human GSK-3β.
Description	Rabbit Monoclonal
Gene	GSK3B
Supplier Page	Shop

Product images

Western blot analysis of extracts from GSK-3α (-/-) (lanes 1,2) and GSK-3β (-/-) (lanes 3,4) mouse embryonic fibroblast (MEF) cells, λ phosphatase or PDGF-treated, using Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb (upper) and α/β-Tubulin Antibody #2148 (lower). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

Western blot analysis of extracts from PC-3 cells, untreated or LY294002/wortmannin-treated, using Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb (upper) or GSK-3β (27C10) Rabbit mAb #9315 (lower).

Confocal immunofluorescent analysis of wild type mouse embryonic fibroblasts (MEFs) (top row), GSK-3β (-/-) MEFs (middle row) , or PC-3 cells (bottom row), untreated (left), LY294002- and Wortmannin-treated (#9901 and #9951 respectively; center) or lambda phosphatase-treated (right), using Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye). (MEF wild type and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or PDGF-treated (green), using Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb.