RM222 specifically reacts to Histone H2A.Z acetylated
at Lysine 7 (K7ac). No cross reactivity with non-modified
Lysine 7 or other acetylated Lysines in histone H2A.
Western Blot of acid extracts from HeLa cells
treated (+) or untreated (-) with sodium butyrate,
using RM222 at 0.5 ug/mL, showed a band of
histone H2A.Z acetylated at Lysine 7 in treated
HeLa.
Immunocytochemical staining of HeLa cells treated
with sodium butyrate, using anti-Acetyl-Histone
H2A.Z (Lys7) Rabbit Monoclonal Antibody (clone
RM222) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).