![RM221 specifically reacts to Histone H2A.Z acetylated<code><br /></code>at Lysine 4 (K4ac). No cross reactivity with non-modified<code><br /></code>Lysine 4 or other acetylated Lysines in histone H2A.<code><br /></code>](http://www.bioprodhub.com/system/product_images/ab_products/95/sub_1/225_RM221_Fig1_E1.png)
RM221 specifically reacts to Histone H2A.Z acetylated
at Lysine 4 (K4ac). No cross reactivity with non-modified
Lysine 4 or other acetylated Lysines in histone H2A.
![Western Blot of acid extracts from HeLa cells<code><br /></code> treated (+) or untreated (-) with sodium butyrate,<code><br /></code>using RM221 at 0.5 ug/mL, showed a band of<code><br /></code>histone H2A.Z acetylated at Lysine 4 in treated<code><br /></code>HeLa.<code><br /></code>](http://www.bioprodhub.com/system/product_images/ab_products/95/sub_1/226_RM221_Fig2-WB_E.png)
Western Blot of acid extracts from HeLa cells
treated (+) or untreated (-) with sodium butyrate,
using RM221 at 0.5 ug/mL, showed a band of
histone H2A.Z acetylated at Lysine 4 in treated
HeLa.
![Immunocytochemical staining of HeLa cells treated<code><br /></code> with sodium butyrate, using anti-Acetyl-Histone <code><br /></code> H2A.Z (Lys4) Rabbit Monoclonal Antibody (clone <code><br /></code> RM221) (red). Actin filaments have been labeled <code><br /></code> with fluorescein phalloidin (green).<code><br /></code>](http://www.bioprodhub.com/system/product_images/ab_products/95/sub_1/426_Fig-3_ICC_H2AZK4ac.png)
Immunocytochemical staining of HeLa cells treated
with sodium butyrate, using anti-Acetyl-Histone
H2A.Z (Lys4) Rabbit Monoclonal Antibody (clone
RM221) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).