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Immunofluorescence of monoclonal antibody to CRKL on HeLa cell . [antibody concentration 10 ug/ml]

Western Blot analysis of CRKL expression in transfected 293T cell line by CRKL monoclonal antibody (M03), clone 4B5.Lane 1: CRKL transfected lysate(33.8 KDa).Lane 2: Non-transfected lysate.

Detection limit for recombinant GST tagged CRKL is approximately 0.3 ng/ml as a capture antibody.

Western blot analysis of CRKL over-expressed 293 cell line, cotransfected with CRKL Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CRKL monoclonal antibody (M03), clone 4B5 . GAPDH ( 36.1 kDa ) used as specificity and loading control.