Glucocorticoid Receptor (D8H2) XP ® Rabbit mAb

Name	Glucocorticoid Receptor (D8H2) XP ® Rabbit mAb
Supplier	Cell Signaling Technology
Catalog	3660
Prices	$109.00, $277.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	D8H2
Applications	WB IP ICC/IF FC ChIP
Species Reactivities	Human, Mouse, Rat, Monkey
Antigen	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu378 of human glucocorticoid receptor protein.
Description	Rabbit Monoclonal
Gene	NR3C1
Supplier Page	Shop

Product images

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human glucocorticoid receptor-α (hGRα-Myc/DDK, +) or Myc/DDK-tagged full-length human mineralocorticoid receptor (hMR-Myc/DDK, +), using Glucocorticoid Receptor (D8H2) XP ® Rabbit mAb (upper) and DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG ® M2 Antibody) #2368 (lower).

Western blot analysis of extracts from various cell lines using Glucocorticoid Receptor (D8H2) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of HeLa cells, grown in phenol red-free media containing 5% charcoal-stripped FBS for 2 days and either untreated (left) or dexamethasone-treated (100 nM, 2hr; right), using Glucocorticoid Receptor (D8H2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Human whole blood was fixed, lysed, and permeabilized as per the Cell Signaling Technology Flow Alternate Protocol and stained using Glucocorticoid Receptor (D8H2) XP ® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody. Samples were gated on CD3+ lymphocytes.

A549 cells were cultured in media with 5% charcoal-stripped FBS for 3 days and then either untreated (left panel) or dexamethasone-treated (100 nM, 1 hr; right panel). Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 cells and 5 µl of Glucocorticoid Receptor (D8H2) XP ® Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human SLC19A2 Promoter Primers #7681, human MT2A promoter primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.