
Western Blot of acid extracts from HeLa cells
untreated (-) or treated with sodium butyrate (+),
using RM179 at 1 ug/mL, showed a band of
histone H3 acetylated at Lysine 56 in treated
HeLa.

RM179 specifically reacts to Histone H3 acetylated at Lysine 56
(K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl),
acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac),
Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36
(K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H3 (Lys56)
Rabbit mAb RM179 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).

Immunohistochemistry staining of HepG2 cells using
Anti-Acetyl-Histone H3 (Lys56) antibody, RM179.