Anti-Acetyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody, Clone RM154

Name	Anti-Acetyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody, Clone RM154
Supplier	RevMab Bioscience
Catalog	31-1050-00
Prices	$149.00, $349.00
Sizes	25 µg, 100 µg
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone	RM154
Applications	WB ELISA Multiplex ICC/IF
Species Reactivities	Broad species
Antigen	An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys36).
Purity/Format	Protein A affinity purified from an animal origin–free culture supernatant
Description	Rabbit Monoclonal
Conjugate	None
Supplier Page	Shop

Western Blot of acid extracts from HeLa cells

untreated (-) or treated with sodium butyrate (+),

using RM154 at 1.0 ug/mL, showed a band of

histone H3 acetylated at Lysine 36 in treated

HeLa.

RM154 specifically reacts to Histone H3 acetylated

at Lysine 36 (K36ac), and is not affected by the

phosphorylation of neighboring Ser31. No cross

reactivity with acetylated Lysine 4 (K4ac), Lysine 9

(K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac),

Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 56

(K56ac), lysine 79 (K79ac), or Lysine 122 (K122)

in histone H3.

Immunocytochemistry of HeLa cells treated with

sodium butyrate, using Acetyl-Histone H3 (Lys36)

Rabbit mAb RM154 (red). Actin filaments have

been labeled with fluorescein phalloidin (green).