RM161 specifically reacts to Histone H3
acetylated at Lysine 9 (K9ac). No cross reactivity
with acetylated Lysine 4 (K4ac), Lysine 14 (K14ac),
Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27
(K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac),
Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.
Western Blot of acid extracts from HeLa cells
untreated (-) or treated with sodium butyrate (+),
using RM161 at 0.25 ug/mL, showed a band of
histone H3 acetylated at Lysine 9 in treated
HeLa.
Sandwich ELISA against acetylated histone H3 at Lys 9
using HeLa whole cell lysate, treated or untreated with
Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as the
capture antibody and biotinylated anti-H3K9ac (RM161,
1 ug/mL) as the detection antibody.
ChIP performed on HeLa cells using H3K9ac antibody (RM161,
5ug). Real-time PCR was performed using primers specific to
the gene indicated.
Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H3 (Lys9)
Rabbit mAb RM161 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).
Immunohistochemistry staining of HepG2 cells using
anti-Acetyl-Histone H3 (Lys9) antibody, RM161.