Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody, Clone RM130 | Antibody search engine

Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody, Clone RM130

Name	Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody, Clone RM130
Supplier	RevMab Bioscience
Catalog	31-1032-00
Prices	$149.00, $349.00
Sizes	25 µg, 100 µg
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone	RM130
Applications	WB ChIP ELISA Multiplex ICC/IF
Species Reactivities	Broad species
Antigen	An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys14)
Purity/Format	Protein A affinity purified from an animal origin–free culture supernatant
Description	Rabbit Monoclonal
Conjugate	None
Supplier Page	Shop

Product images

RM130 specifically reacts to Histone H3 acetylated <code><br /></code> at Lysine 14 (K14ac). No cross reactivity with <code><br /></code> acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine <code><br /></code>18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), <code><br /></code> Lysine 36 (K36ac), or lysine 79 (K79ac) in histone <code><br /></code> H3.<code><br /></code>

RM130 specifically reacts to Histone H3 acetylated

at Lysine 14 (K14ac). No cross reactivity with

acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine

18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac),

Lysine 36 (K36ac), or lysine 79 (K79ac) in histone

H3.

The specific binding of RM130 to Histone H3 acetylated <code><br /></code> at Lysine 14 (K14ac) is not affected by the modification<code><br /></code> of neighboring amino acids.<code><br /></code>

The specific binding of RM130 to Histone H3 acetylated

at Lysine 14 (K14ac) is not affected by the modification

of neighboring amino acids.

Western Blot of acid extracts from HeLa cells untreated (-) <code><br /></code> or treated with sodium butyrate (+), using RM130<code><br /></code>at 0.5 ug/mL, showed a band of histone H3 acetylated <code><br /></code>at Lysine 14 in treated HeLa.<code><br /></code>

Western Blot of acid extracts from HeLa cells untreated (-)

or treated with sodium butyrate (+), using RM130

at 0.5 ug/mL, showed a band of histone H3 acetylated

at Lysine 14 in treated HeLa.

ChIP performed on HeLa cells using H3K14ac <code><br /></code>antibody (RM130, 5ug). Real-time PCR was<code><br /></code> performed using primers specific to the gene indicated.<code><br /></code>

ChIP performed on HeLa cells using H3K14ac

antibody (RM130, 5ug). Real-time PCR was

performed using primers specific to the gene indicated.

Immunocytochemistry of HeLa cells treated with<code><br /></code>sodium butyrate, using Acetyl-Histone H3 (Lys14) <code><br /></code>Rabbit mAb RM130 (red). Actin filaments have<code><br /></code>been labeled with fluorescein phalloidin (green).<code><br /></code>

Immunocytochemistry of HeLa cells treated with

sodium butyrate, using Acetyl-Histone H3 (Lys14)

Rabbit mAb RM130 (red). Actin filaments have

been labeled with fluorescein phalloidin (green).