![RM149 specifically reacts to Histone H3 acetylated<code><br /></code>at Lysine 4 (K4ac). No cross reactivity with non-<code><br /></code>modified Lysine 4, acetylated Lysine 9 (K9ac), Lysine<code><br /></code>14 (K14ac), Lysine 18 (K18ac),Lysine 23 (K23ac), <code><br /></code> Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56<code><br /></code> (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in<code><br /></code>histone H3.<code><br /></code>](http://www.bioprodhub.com/system/product_images/ab_products/95/sub_1/66_Fig1_RM149_E.png)
RM149 specifically reacts to Histone H3 acetylated
at Lysine 4 (K4ac). No cross reactivity with non-
modified Lysine 4, acetylated Lysine 9 (K9ac), Lysine
14 (K14ac), Lysine 18 (K18ac),Lysine 23 (K23ac),
Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56
(K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in
histone H3.
![Western Blot of acid extracts from HeLa cells <code><br /></code> untreated (-) or treated with sodium butyrate (+),<code><br /></code> using RM149 at 0.5 ug/mL, showed a band of <code><br /></code>histone H3 acetylated at Lysine 4 in treated<code><br /></code> HeLa.<code><br /></code>](http://www.bioprodhub.com/system/product_images/ab_products/95/sub_1/67_Fig2_RM149_WB.png)
Western Blot of acid extracts from HeLa cells
untreated (-) or treated with sodium butyrate (+),
using RM149 at 0.5 ug/mL, showed a band of
histone H3 acetylated at Lysine 4 in treated
HeLa.
![Immunocytochemistry of HeLa cells treated with<code><br /></code>sodium butyrate, using Acetyl-Histone H3 (Lys4) <code><br /></code>Rabbit mAb RM149 (red). Actin filaments have<code><br /></code>been labeled with fluorescein phalloidin (green).<code><br /></code>](http://www.bioprodhub.com/system/product_images/ab_products/95/sub_1/429_Fig3a_RM149_ICC.png)
Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H3 (Lys4)
Rabbit mAb RM149 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).