
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

The anti-Phospho-CHK1-S280 antibody is used in Western blot to detect Phospho-CHK1-S280 in, from left to right, A2058, mouse brain, Ramos, Jurkat, HL60, and HeLa tissue lysates.