Western blot analysis of extracts from Jurkat and PC3 cells using FoxO3a (75D8) Rabbit mAb.
Western blot analysis of extracts from wild type (+/+) and FoxO3 knock-out (-/-) mouse brain, using FoxO3a (75D8) Rabbit mAb (upper) and FoxO1 (C29H4) Rabbit mAb #2880 (lower). (FoxO3 knock-out (-/-) mouse brain was kindly provided by Dr. Ron DePinho, Dana-Farber Cancer Institute, Boston, MA).
Confocal immunofluorescent analysis of SH-SY5Y cells, IGF-I treated (left) or LY294002-treated, using FoxO3a (75D8) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).