Immunofluorescence of CA9 Antibody with HeLa cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
Western blot of CA9 antibody in HeLa cell line lysates (35 ug/lane). CA9 (arrow) was detected using the purified antibody.
Flow cytometric of HeLa cells using CA9 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.