RIPAb+ AUF1 - RIP Validated Antibody and Primer Set

Name	RIPAb+ AUF1 - RIP Validated Antibody and Primer Set
Supplier	EMD Millipore
Catalog	03-111
Prices	$409.00
Sizes	10 assays 10 assays per set. recommended use: ~5 μg of antibody per rip . (dependent upon biological context)
Host	Rabbit
Clonality	Polyclonal
Applications	WB RIP-Chip IP
Species Reactivities	Rat, Human, Mouse
Antigen	Human AUF1 purified by Ni 2+ affinity column.
Description	Rabbit Polyclonal
Gene	HNRNPD
Supplier Page	Shop

Product images

RNA Binding Protein Immunoprecipitation:<br />RIP Lysate prepared from HeLa cells (2 X 107 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG (Part # PP64B), or 5 µg of Anti-AUF1 antibody (Part # CS204387) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).<br />Successful immunoprecipitation of AUF1-associated RNA was verified by qPCR using RIP Primers FOS, (Part # CS204344) (Figure 1). <br />Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.<br />

RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (2 X 107 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG (Part # PP64B), or 5 µg of Anti-AUF1 antibody (Part # CS204387) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of AUF1-associated RNA was verified by qPCR using RIP Primers FOS, (Part # CS204344) (Figure 1).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.