![Western blot of extracts (35 ug) from 9 different cell lines by using anti-PIK3AP1 monoclonal antibody.](http://www.bioprodhub.com/system/product_images/ab_products/3/sub_1/13521_127072_60391.jpg)
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PIK3AP1 monoclonal antibody.
![HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIK3AP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIK3AP1.](http://www.bioprodhub.com/system/product_images/ab_products/3/sub_1/13522_127071_60390.jpg)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIK3AP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIK3AP1.
![Flow cytometry of Jurkat cells, using anti-PIK3AP1 antibody (Red), compared to a nonspecific negative control antibody (Blue).](http://www.bioprodhub.com/system/product_images/ab_products/3/sub_1/13523_127074_60393.jpg)
Flow cytometry of Jurkat cells, using anti-PIK3AP1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
![HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PIK3AP1 antibody, and then analyzed by flow cytometry.](http://www.bioprodhub.com/system/product_images/ab_products/3/sub_1/13524_127073_60392.jpg)
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PIK3AP1 antibody, and then analyzed by flow cytometry.