EGR1 (15F7) Rabbit mAb

Name	EGR1 (15F7) Rabbit mAb
Supplier	Cell Signaling Technology
Catalog	4153
Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	15F7
Applications	WB IP IHC-P ICC/IF ChIP
Species Reactivities	Human, Mouse, Rat, Bovine
Antigen	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of the sequence of human EGR1.
Description	Rabbit Monoclonal
Gene	EGR1
Supplier Page	Shop

Product images

Western analysis of extracts from serum starved PC12 cells, untreated or NGF-treated (100 ng/ml NGF for 2 hours), using EGR1 (15F7) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse brain using EGR1 (15F7) Rabbit mAb in the presence of control peptide (left) or EGR1 (15F7) Blocking Peptide #1015 (right).

Immunohistochemical analysis of paraffin-embeded human glioblastoma showing nuclear localization, using EGR1 (15F7) Rabbit mAb.

Immunohistochemical analysis of paraffin-embeded human Non-Hodgkin's lymphoma, using EGR1 (15F7) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse brain, using EGR1 (15F7) Rabbit mAb.

Confocal immunofluorescent analysis of dissociated PC12 cells either untreated (left) or NGF-treated for 2 hours (right) and labeled with EGR1 (15F7) Rabbit mAb (green) and MEK1/2 (L38C12) Mouse mAb #4694 (red).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 THP-1 cells treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (30ng/ml) overnight and either 10 μl of EGR1 (15F7) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ATAD2 promoter primers, SimpleChIP® Human EGR1 Promoter Primers #5549, human PCM1 promoter primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.