
p-Pol II (8A7): sc-13583. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

p-Pol II (8A7): sc-13583. Western blot analysis of Pol II phosphorylation in untreated (A), heat shocked (C) and calyculin-treated (E) HeLa cultures. Total Pol II levels were determined in the same respective extracts by using Pol II (A-10): sc-17798 (B,D,F).

p-Pol II (8A7): sc-13583. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

p-Pol II (8A7): sc-13583. Western blot analysis of Pol II phosphorylation in untreated (A) and lambda protein phosphatase treated (B) HeLa whole cell lysates.

Western blot analysis of Pol II phosphorylation in untreated (A,D), calyculin A treated (B,E) and calyculin A and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-Pol II (8A7): sc-13583 (A,B,C) and Pol II (8WG16): sc-56767 (D,E,F).