![HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANKRD53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANKRD53.](http://www.bioprodhub.com/system/product_images/ab_products/3/sub_1/6633_114514_23476.jpg)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANKRD53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANKRD53.
![Flow cytometry of Jurkat cells, using anti-ANKRD53 antibody (Red), compared to a nonspecific negative control antibody (Blue).](http://www.bioprodhub.com/system/product_images/ab_products/3/sub_1/6634_114517_23479.jpg)
Flow cytometry of Jurkat cells, using anti-ANKRD53 antibody (Red), compared to a nonspecific negative control antibody (Blue).
![Flow cytometry of HeLa cells, using anti-ANKRD53 antibody (Red), compared to a nonspecific negative control antibody (Blue).](http://www.bioprodhub.com/system/product_images/ab_products/3/sub_1/6635_114516_23478.jpg)
Flow cytometry of HeLa cells, using anti-ANKRD53 antibody (Red), compared to a nonspecific negative control antibody (Blue).
![HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ANKRD53 antibody, and then analyzed by flow cytometry.](http://www.bioprodhub.com/system/product_images/ab_products/3/sub_1/6636_114515_23477.jpg)
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ANKRD53 antibody, and then analyzed by flow cytometry.